Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 306, Issue 1, Pages 213-218Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S0006-291X(03)00935-5
Keywords
DNA hybridization; metal-enhanced fluorescence; silver nanoparticles
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Funding
- NCRR NIH HHS [RR-08119, P41 RR008119-14, P41 RR008119, P41 RR008119-13] Funding Source: Medline
- NHGRI NIH HHS [R01 HG002655-06, HG-002655, R01 HG002655-03, R01 HG002655-05, R01 HG002655] Funding Source: Medline
- NIBIB NIH HHS [EB-00981, R01 EB000682, R01 EB000682-04, R21 EB000981-01, EB-000682, R21 EB000981-02, R21 EB000981, R01 EB000682-05] Funding Source: Medline
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We describe a new approach to DNA hybridization assays using metal-enhanced fluorescence. Thiolated oligonucleotides were bound to silver particles on a glass substrate. Addition of a complementary fluorescein-labeled oligonucleotide resulted in a dramatic time-dependent 12-fold increase in fluorescence intensity during hybridization. Proximity to silver particles resulted in a decreased fluorescence lifetime. This effect is thought to be the result of enhanced fluorescence from fluorescein near metallic silver particles. Hybridization could thus be measured from the decay kinetics of the emission, which can be measured independently from the emission intensity. These results suggest the use of silver particles as a general approach to measure DNA hybridization as a method to increase the sensitivity of DNA detection. (C) 2003 Elsevier Science (USA). All rights reserved.
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