Journal
VIROLOGY
Volume 311, Issue 1, Pages 181-191Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S0042-6822(03)00190-9
Keywords
Flock House virus; chimeric RNA; replication signals; RNA heterodimers; RNA replication; transactivation
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Funding
- NIAID NIH HHS [R01AI18270] Funding Source: Medline
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The nodavirus Flock House virus has a bipartite positive-sense RNA genome consisting of RNAs I and 2, which encode the viral RNA-dependent RNA polymerase (RdRp) and capsid protein precursor, respectively. The RdRp catalyzes replication of both genome segments and produces from RNA1 a subgenomic RNA (RNA3) that transactivates RNA2 replication. Here, we replaced internal sequences of RNAs 1 and 2 with a common heterologous core and were thereby able to test the RNA termini for compatibility in supporting the replication of chimeric RNAs. The results showed that the 3' 50 nt of RNA2 contained an RNA3-dependent cis-acting replication signal. Since covalent RNA dimers can direct the synthesis of monomeric replication products, the RdRp can evidently respond to cis-acting replication signals located internally. Accordingly, RNA templates containing the 3' termini of both RNAs 1 and 2 in tandem generated different replication products depending on the presence or absence of RNA3. (C) 2003 Elsevier Science (USA). All rights reserved.
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