Journal
ARHIV ZA HIGIJENU RADA I TOKSIKOLOGIJU-ARCHIVES OF INDUSTRIAL HYGIENE AND TOXICOLOGY
Volume 63, Issue 1, Pages 15-20Publisher
INST MEDICAL RESEARCH & OCCUPATIONAL HEALTH
DOI: 10.2478/10004-1254-63-2012-2132
Keywords
CCO cells; HeLa cells; imidazolium ionic liquids; MTT assay
Funding
- Ministry of Science, Education and Sports of the Republic of Croatia [058-0582184-2414, 058-0582261-2256]
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Ionic liquids bring a promise of a wide range of green applications that could replace conventional volatile solvents. However, before these applications become large-scale, their toxicity needs to be investigated in order to predict the impact on human health and environment. In this study we assessed the cytotoxicity of imidazolium ionic liquids (in the concentrations between 0.1 mmol L-1 and 10 mmol L-1) in the ovarian fish cell line CCO and the human tumour cell line HeLa using the MTT cell viability assay. Our results showed that the most cytotoxic ionic liquid was 1-n-butyl-3-methylimidazolium bis(trifluoro methylsulphonyl)imide, [BMIK[Tf2N], followed by 1-n-butyl-3-methylimidazolium tetrafluoroborate [BMIM][BF,), 1-n-butyl-3-methylimidazolium hexafluorophosphate [BMIM][PF6], and 1,3-dimethylitnidazolium hexafluorophosphate [MMIM][PF6]. Generally, the effects were concentration-dependent. They also depended on the type of anion and the n-alkyl chain length. The comparison between the fish CCO and human HeLa cell lines suggests that CCO cells provide a good biological system for initial toxicity testing of ionic liquids that could replace in vivo bioassays.
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