Journal
TOXICOLOGY
Volume 188, Issue 2-3, Pages 261-274Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/S0300-483X(03)00089-1
Keywords
apoptosis; cell differentiation; dehydrocrotonin; dimethylamide-crotonin; HL60 cells; human peripheral blood mononuclear
Categories
Ask authors/readers for more resources
Dehydrocrotonin (DHC) is a diterpene lactone obtained from Croton cajucara (Sacaca). Dimethylamide-crotonin (DCR), a DHC derivative, has a similar inhibitory effect on leukemic HL60 cells than its parent compound evaluated by different endpoints of cytotoxicity. No cytotoxicity or morphological alterations associated with apoptosis were detected in human peripheral blood mononuclear cells (PBMC) after treatment with up to 400 muM DCR in presence of phytohemaglutinin (5 mug/ml). Based on morphological changes and the pattern of DNA fragmentation, DHC and DCR were found to induce apoptosis and terminal differentiation (assessed by nitro blue tetrazolium reduction) in HL60 cells, but these compounds did not show any toxic effect in PBMC. Thus, DCR and DHC inhibit HL60 cell growth in vitro partly by inducing apoptosis and cell differentiation, but does not cause serious damage to immune cells according to our experimental conditions. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available