Journal
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
Volume 56, Issue 1-3, Pages 265-279Publisher
ELSEVIER
DOI: 10.1016/S0165-022X(03)00064-2
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Size exclusion of cellulose in LiCl/N,N-dimethylacetamide has been used for the past 15 years, yet much of the current research is still devoted to fundamental studies, as many issues regarding column calibration, separation mechanisms and solution behavior have not been resolved yet. The paper reviews practical aspects of sample preparation and it is demonstrated that sample heating and several techniques to aid solvent exchange call for reevaluation. It is further shown that the use of internal standard may introduce minor improvements in repeatability. The commonly used column calibration procedures, chromatographic conditions and applications are also reviewed. Further research is needed to understand the mechanisms of separation, to optimize column calibration and to facilitate and optimize sample preparation. (C) 2003 Elsevier Science B.V. All rights reserved.
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