Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 82, Issue 7, Pages 778-783Publisher
JOHN WILEY & SONS INC
DOI: 10.1002/bit.10635
Keywords
Ramy3D; recombinant human GM-CSF; transgenic rice cell suspension culture
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Recombinant human granulocyte-macrophage colony stimulating factor (hGM-CSF) has been previously produced in tobacco cell suspension cultures. However, the amount of hGM-CSF accumulated in the culture medium dropped quickly from its maximum of 150 mug/L at 5 d after incubation. To overcome this problem, we sought an expression system in which heterologous gene expression could be induced at high levels. We selected a rice amylase expression system in which the promoter Ramy3D is induced to express recombinant protein by sucrose starvation. This induction system was found to give good yield of recombinant hGM-CSF in transgenic rice cell suspension culture and protease activity of this culture medium was low compared to that of tobacco culture system. (C) 2003 Wiley Periodicals, Inc.
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