Journal
RNA
Volume 9, Issue 7, Pages 802-808Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.5270203
Keywords
RNAi; siRNAs; polyribosomes; ribonucleoprotein; Trypanosoma brucei
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Funding
- NIAID NIH HHS [R37 AI028798, R01 AI028798, AI28798] Funding Source: Medline
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Genetic interference by double-stranded RNA (dsRNA) or RNA interference (RNAi) triggers the sequence-specific degradation of cellular transcripts. The mediators of mRNA degradation are small interfering RNAs (siRNAs). Here, we report that in Trypanosoma brucei, 10%-20% of siRNAs cosediment with polyribosomes. Preventing the assembly of ribosomes on mRNAs results in a concomitant decrease of siRNAs sedimenting as high-molecular-weight complexes. We further provide evidence that siRNAs are associated with translating ribosomes and that this association is mediated by a 70-kD ribonucleoprotein complex.
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