Journal
JOURNAL OF IMMUNOTHERAPY
Volume 26, Issue 4, Pages 332-342Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/00002371-200307000-00005
Keywords
adoptive cell transfer; tumor-infiltrating lymphocyte; melanoma; tumor antigen; immunotherapy
Categories
Funding
- Intramural NIH HHS [Z01 SC003811-32] Funding Source: Medline
Ask authors/readers for more resources
The generation of T lymphocytes with specific reactivity against tumor antigens is a prerequisite for effective adoptive transfer therapies. Melanoma-specific lymphocyte cultures can be established from tumor infiltrating lymphocytes (TILs) by in vitro culture in high levels of IL-2. We have optimized methods for generating melanoma-reactive TIL cultures from small resected tumor specimens. We report a retrospective analysis of 860 attempted TIL cultures from 90 sequential melanoma biopsy specimens from 62 HLA-A2(+) patients. Multiple independent TIL derived from a single tumor often exhibited substantial functional and phenotypic variation. Tumor specific activity was detected in TIL from 29 (81%) of 36 patients screened. TIL cultures selected for high activity were generally capable of large numerical expansion using a single round of a rapid expansion protocol. Limited clonal T-cell populations in an oligoclonal TIL culture could confer specific tumor recognition in these highly selected, highly expanded TIL cultures. These methods were efficient at generating TILs suitable for adoptive transfer therapy.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available