The interaction of endochitinase, a synthetic peptide and resveratrol in controlling fungi in vitro

The efficacy of two-way combinations of Trichoderma endochitinase (CHIT42), synthetic peptide ESF12 and resveratrol upon the control of growth of Botrytis cinerea and Penicillium digitatum were evaluated in vitro. CHIT42 had effective concentration for 50% growth inhibition (EC50) values of 1.5 and 0.5 muM against B. cinerea and P digitatum, respectively. CHIT42 also caused swelling, hyperbranching and bursting of P digitatum at concentrations as low as 0.003 muM. The EC50 values of ESF12 were 30.9 and 29.2 muM against B. cinerea and R digitatum, respectively. Resveratrol had EC50 values of 178.6 and 343.2 muM against B. cinerea and P digitatum, respectively. Combinations of CHIT42 + ESF12, CHIT42 + resveratrol and ESF12 + resveratrol showed additive activity (interaction ratios between 0.5 and 1.5) against B. cinerea and P digitatum.