Journal
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR
Volume 75, Issue 4, Pages 769-776Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0091-3057(03)00152-7
Keywords
Autoreceptor; depression; stress; tail shock; restraint; forced swim
Funding
- NIDA NIH HHS [DA14545] Funding Source: Medline
- NIMH NIH HHS [MH63303] Funding Source: Medline
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Serotonergic neurons in the dorsal raphe nucleus, the major source of forebrain serotonin projections, synthesize a terminal autoreceptor that inhibits serotonin release-the 5-HT1B autoreceptor. Overexpression of this autoreceptor is hypothesized to contribute to anxiety. Antidepressants decrease (while learned helplessness increases) 5-HT1B mRNA in dorsal raphe neurons, and viral-mediated overexpression of 5-HT1B here increases anxiety behavior after stress. However, 5-HT1B mRNA levels in dorsal raphe are substantially elevated in unstressed rats in two models of stress resistance. Thus, the role of dorsal raphe 5-HT1B autoreceptors in anxiety is complex. Therefore, we tested whether different stressors differentially affect dorsal raphe 5-HT1B mRNA [via in situ hybridization histochemistry] and anxiety behavior (using the elevated plus maze). Rats were assigned to a stressor (either forced swim, water restraint, dry restraint, or electric tail shock) or a control condition, then were tested and sacrificed 24 It later. Overall, controls exhibited less anxiety than stressed rats as indicated by a higher ratio of open arm to total arm entries (OTR). The stressors did not difterentially affect the OTR, nor did any alter dorsal raphe 5-HT1B mRNA levels. There was, however, a significant positive correlation between the OTR and 5HT(1B) mRNA intensity in controls (r=.64; P=.006), but not in stressed rats (r=.16, P=.36), providing further evidence that elevated dorsal raphe 5-HT1B levels are associated with reduced anxiety in animals that have not been exposed to stress. (C) 2003 Elsevier Inc. All rights reserved.
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