Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 8, Issue 3, Pages 362-367Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.1577574
Keywords
fluorescence lifetime imaging microscopy
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Fluorescence lifetime imaging microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. Recent developments in multiphoton FLIM methods are reviewed and a novel multiphoton FLIM system using a streak camera is described. An example of a typical application of the system is provided in which the fluorescence resonance energy transfer between a donor-acceptor pair of fluorescent proteins within a cellular specimen is measured. (C) 2003 Society of Photo-Optical Instrumentation Engineers.
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