Journal
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
Volume 52, Issue 1, Pages 61-64Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jac/dkg268
Keywords
erythromycin; moxifloxacin; penicillin; tetracycline; chloramphenicol
Funding
- NIAID NIH HHS [AI35257] Funding Source: Medline
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The effect of antimicrobial concentration on colony-forming ability of resistant mutant subpopulations of Mycobacterium smegmatis and Staphylococcus aureus was measured for chloramphenicol, erythromycin, moxifloxacin, penicillin and tetracycline. The relationship between drug concentration and the recovery of mutant colonies was distinct for each bacterium-antimicrobial combination; however, in each case application of large numbers of cells to drug-containing agar plates revealed a progressive reduction in mutant recovery as antimicrobial concentration increased. The minimal concentration that allowed no mutant recovery from more than 10(10) input cells was measured to estimate the minimum inhibitory concentration (MIC) of the least susceptible, single-step mutant subpopulation, a parameter also called the mutant prevention concentration (MPC). These data expand the number of antimicrobial-bacterial combinations for which a mutant selection window can be measured.
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