Journal
PHYTOCHEMICAL ANALYSIS
Volume 14, Issue 4, Pages 224-227Publisher
WILEY
DOI: 10.1002/pca.707
Keywords
differential pulse polarography; thymoquinone; black seed oil; Nigella sativa
Ask authors/readers for more resources
A reliable and simple differential pulse polarographic method is described for the determination of thymoquinone in black seed oil. The polarographic behaviour of thymoquinone was examined in various buffer systems over the pH range 5.0-10.0. Thymoquinone is reduced in a single, reversible peak at the dropping mercury electrode. The differential pulse polarogram showed a distinct peak in Sorensen buffer:methanol (3:7, v/v; pH 8.5) at a peak potential of -0.095 V (vs. silver/silver chloride electrode), and a plot of peak height against concentration was found to be linear over the range 0.2-15.0 mug/mL (R = 0.9998). The limit of detection was calculated to be 0.054 mug/mL. The polarographic method has been applied to determine thymoquinone in two black seed oil preparations available on the Austrian pharmaceutical market. Copyright (C) 2003 John Wiley Sons, Ltd.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available