Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 69, Issue 7, Pages 4243-4248Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.69.7.4243-4248.2003
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Bacterial proliferations have recurrently been observed for the past 15 years in fermentor cultures of the ectomycorrhizal fungus Laccaria bicolor S238N, suggesting the presence of cryptic bacteria in the collection culture of this fungus. In this study, intracellular bacteria were detected by fluorescence in situ hybridization in combination with confocal laser scanning microscopy in several collection subcultures of L. bicolor S238N. They were small (0.5 mum in diameter), rare, and heterogeneously distributed in the mycelium and were identified as Paenibacillus spp. by using a 16S rRNA-directed oligonucleotide probe initially designed for bacteria isolated from a fermentor culture of L. bicolor S238N.
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