Relationships between leaf-blade nitrogen and relative seedcotton yields

Nitrogen fertilization is a required production practice for cotton (Gossypium hirsutum L.) with risks arising from under- and over-fertilization. Tissue testing for diagnosing N deficiencies in crops can use leaf blades and the total N concentration, but this practice has not been rigorously examined in cotton. The primary objective of these experiments was to determine the leaf-N concentration of the uppermost, fully mature leaf blade below which yield loss could be expected. Nitrogen-rate field experiments were conducted at 12 research station and farm sites in the Midsouth USA in Louisiana, Arkansas, Mississippi, and Alabama in 1996 and 1997. Leaf-blade total N concentrations associated with yield loss were 4.3% N at early bloom (R-2 = 0.50) and 4.1% N at mid-bloom (3 wk after early bloom, R-2 = 0.32). The likelihood of applying N when not needed could be reduced by lowering the early bloom critical value to 3.9%. Only 4% of all samples sufficient in N would have been incorrectly diagnosed N deficient at that critical value, but 44% of all deficient samples would have been misidentified as N sufficient. Reduced yields due to over application of N were evident in some samples with leaf N between 4.6 and 4.8% at early bloom. These concentrations were also common for N-sufficient plants, making accurate diagnoses of the over application of N unlikely. Our leaf-N critical values probably differ from previously established values because earlier values were derived via survey techniques and because faster fruiting cultivars may require higher leaf N.