Journal
MOLECULAR ECOLOGY
Volume 12, Issue 7, Pages 2011-2017Publisher
WILEY
DOI: 10.1046/j.1365-294X.2003.01874.x
Keywords
cryptic species; diagnostic DNA methods; Microtus; single base extension; single nucleotide polymorphisms (SNP)
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There is growing interest in the use of single nucleotide polymorphisms for evolutionary and population genetics. We tested the efficacy of one of the available single nucleotide polymorphism techniques, single-base extension, in distinguishing four cryptic species of Microtus . Sequence data were available for these species at nuclear and mitochondrial loci and their identity could be independently confirmed using karyotypes. We found that the development and optimization of single nucleotide polymorphisms required extensive effort, and that the method accurately identified the correct nucleotide at single nucleotide polymorphism sites approximate to 90% of the time at the conserved nuclear locus. Correct identification rates were much lower at the highly variable mitochondrial locus.
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