Journal
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
Volume 53, Issue 3, Pages 125-133Publisher
WILEY-LISS
DOI: 10.1002/arch.10091
Keywords
insect immunity; antifungal peptide; gallerimycin
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A novel defensin-like peptide was identified in the greater wax moth, Galleria mellonella. it was discovered in a haemocyte cDNA bank enriched with transcripts upregulated after immune challenge via subtractive hybridisation and suppressive PCR. The deduced amino acid sequence of the defensin-like peptide exhibits similarities to the antifungal peptides drosomycin from Drosophila melonogaster and heliomicin from Heliothis virescens. Therefore, it has been termed gallerimycin. Upregulation of gallerimycin after stimulation of the immune system by LPS-injection was demonstrated by quantitative real-time PCR. A full-size cDNA was cloned and overexpressed in Escherichia coli Origami cells in order to obtain a functional peptide with disulfide bridges. The recombinant peptide was active against the entomopathogenic fungus Metarhizium anisopliae, but not against yeast, gram-negative and gram-positive bacteria. (C) 2003 Wiley-Liss, Inc.
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