Journal
ARCHIVES OF VIROLOGY
Volume 163, Issue 11, Pages 3119-3124Publisher
SPRINGER WIEN
DOI: 10.1007/s00705-018-3967-7
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Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2015R1D1A1A09057406]
- National Research Foundation of Korea [2015R1D1A1A09057406] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The present study was conducted to examine whether cellular and/or viral cholesterol levels play a role in porcine delta-coronavirus (PDCoV) replication. Our results showed that depletion of cholesterol from cells or virions by treating them with methyl-beta-cyclodextrin (M beta CD) diminished PDCoV infection in a dose-dependent manner. The addition of exogenous cholesterol to M beta CD-treated cells or virions moderately restored PDCoV infectivity. Furthermore, the pharmacological sequestration of cellular or viral cholesterol efficiently blocked both virus attachment and internalization. Taken together, the current data indicate that the cholesterol present in the cell membrane and viral envelope contributes to PDCoV replication by acting as a key component in viral entry.
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