Journal
ARCHIVES OF VIROLOGY
Volume 159, Issue 5, Pages 885-896Publisher
SPRINGER WIEN
DOI: 10.1007/s00705-013-1860-y
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Funding
- Grants-in-Aid for Scientific Research [12J07554, 24688005, 11J06256] Funding Source: KAKEN
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Plant virus expression vectors provide a powerful tool for basic research as well as for practical applications. Here, we report the construction of an expression vector based on plantago asiatica mosaic virus (PlAMV), a member of the genus Potexvirus. Modification of a vector to enhance the expression of a foreign gene, combined with the use of the foot-and-mouth disease virus 2A peptide, allowed efficient expression of the foreign gene in two model plant species, Arabidopsis thaliana and Nicotiana benthamiana. Comparison with the widely used potato virus X (PVX) vector demonstrated that the PlAMV vector retains an inserted foreign gene for a longer period than PVX. Moreover, our results showed that the GFP expression construct PlAMV-GFP exhibits stronger RNA silencing suppression activity than PVX-GFP, which is likely to contribute to the stability of the PlAMV vector.
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