Journal
ARCHIVES OF VIROLOGY
Volume 158, Issue 10, Pages 2153-2156Publisher
SPRINGER WIEN
DOI: 10.1007/s00705-013-1706-7
Keywords
-
Categories
Funding
- National Science Centre of Poland [UMO-2011/01/D/NZ9/00279]
Ask authors/readers for more resources
Loop-mediated isothermal amplification (LAMP) of DNA is a novel technique for the amplification of DNA under isothermal conditions. For the first time, we applied this method to develop a simple and sensitive tool for the detection of Pepino mosaic virus (PepMV). PepMV is an emerging pathogen that causes yield and quality losses in tomato crops. Specific RT-LAMP primers for PepMV detection were designed based on triple gene block sequences. The reaction was performed in a single tube at 65 A degrees C for 30 min. The RT-LAMP assay is easy to perform and inexpensive, and it may be applied in the rapid and specific diagnosis of PepMV.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available