Journal
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
Volume 26, Issue 4, Pages 251-260Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0147-9571(02)00054-1
Keywords
equine arteritis virus; c-ELISA; monoclonal antibodies; anti-peptide serum
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In an effort to further characterize the humoral immune response of horses to equine arteritis virus (EAV), direct and competitive enzyme-linked immunosorbent assays (c-ELISAs) were developed using monoclonal and polyclonal anti-sera to structural (G(L), N and M) and non-structural (nsp1) viral proteins. A nsp1-specific monoclonal antibody was produced to facilitate development of a c-ELISA to this protein. Data obtained using the various c-ELISAs confirm that the M protein is a major target of the antibody response of horses to EAV. However, none of the c-ELISAs that were developed were as sensitive in detecting EAV-specific antibodies in horse sera as the existing serum neutralization test. (C) 2003 Elsevier Science Ltd. All rights reserved.
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