Journal
EUROPEAN JOURNAL OF OBSTETRICS GYNECOLOGY AND REPRODUCTIVE BIOLOGY
Volume 109, Issue 1, Pages 8-15Publisher
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0301-2115(02)00350-0
Keywords
lymphocyte; cytokine; decidua; preeclampsia
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Background: Excessive Th I activity in peripheral blood plays a probable role in the pathogenesis of preeclampsia. The aim of the study was to investigate whether disturbed local immune reactions are also present in decidua. Methods: Flow cytometric analysis of CD3, CD19, CD56/ CD16, CD4. CD8, CD4/CD29, CD4/CD45RA, CD4/CD45RO, CD8/CD28, CD3/CD69 lymphocyte subsets isolated from third trimester decidua of pregnants with preeclampsia (n = 21) and pregnant controls (n = 11) subjected to elective caesarean sections. Spontaneous and phytohemaglutynine stimulated in vitro secretion of IL-2, IL-4, IL-6, IL-10,IL-12, IFN-gamma and TGF-beta by decidual lymphocytes was studied by ELISA. For the statistical significance of differences between the groups the U Mann-Whitney test was performed (confidence interval P < 0.05). Results: Preeclamptic patients were characterized with an increased percentage of the CD3-/CD56+CD16+, CD8+/CD28+ and decreased percentage of CD3+, CD19+, CD4+/CD45RA+ lymphocytes. The profile of secreted cytokines shifts in favor of Th I activity (extremely high IFN-gamma and low IL-6 and IL-10 secretion). Decidual IL-12 secretion in preeclamptic patients is decreased compared to controls. Conclusion: Changes in NK and T lymphocyte subsets followed with Th I cytokine IFN-gamma over-activity, could affect local immunoregulatory mechanisms in third trimester decidua of preeclamptic patients. (C) 2002 Published by Elsevier Science Ireland Ltd.
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