Journal
ARCHIVES OF VIROLOGY
Volume 157, Issue 11, Pages 2213-2217Publisher
SPRINGER WIEN
DOI: 10.1007/s00705-012-1376-x
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Funding
- Special Fund for Agro-scientific Research in the Public Interest [200903049]
- Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) [IRT1042]
- National Natural Science Foundation of China [31070535]
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Cucumber mosaic virus (CMV) is one of the most devastating threats to the banana industry. A single-tube, one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid detection of CMV-infected banana and plantain (Musa spp.). The reaction was performed in a single tube at 63 A degrees C for 90 min using a real-time turbidimeter, with an improved closed-tube visual detection system in which fluorescent dye was added to the inside of the lid prior to amplification. This RT-LAMP assay is an alternative method for the rapid detection of CMV in banana plants and tissue culture materials.
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