Journal
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
Volume 50, Issue 4, Pages 492-497Publisher
PLEIADES PUBLISHING INC
DOI: 10.1023/A:1024768622970
Keywords
Panax ginseng; ribonucleases; PR-10 proteins; ethylene; methyl jasmonate; H-7 inhibitor of kinases
Categories
Ask authors/readers for more resources
Cultured ginseng cells (Panax ginseng C.A. Mey strain R-1) produce two proteins exhibiting RNase activity (Pg1 and Pg2), which, on the basis of their amino acid sequences, have been earlier referred to intracellular pathogenesis-related proteins. An immunoenzyme technique for estimation of these proteins was developed. A close correlation was found between the content of these proteins and the RNase activity of the cultured cells. Ethephon and jasmonic acid activated the RNase activity, ethephon being more efficient. Salicylic acid did not activate Pg1 and Pg2; high concentrations of salicylic acid suppressed the RNase activity of the culture. The protein kinase inhibitor, H-7, reduced the content and activity of RNases both in the presence and absence of ethephon. The results obtained permit a suggestion that ethylene and jasmonic acid signaling pathways, which include protein phosphorylation, are involved in the induction of PR-10 proteins.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available