Journal
ARCHIVES OF VIROLOGY
Volume 158, Issue 3, Pages 673-678Publisher
SPRINGER WIEN
DOI: 10.1007/s00705-012-1519-0
Keywords
LTSV; TRoV; Sobemovirus; Genome sequencing; N-terminal transmembrane anchor
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Funding
- EU through the European Regional Development Fund (Center of Excellence ENVIRON)
- Estonian Science Foundation [9415]
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Unlike other sobemoviruses, lucerne transient streak virus (LTSV) and turnip rosette virus (TRoV) have been reported to contain two successive ORF1s (denoted as ORF1a and ORF1b) instead of a single ORF1. Also, their next ORF (ORF2a/2a2b) has been mapped to a region ca. 200 nucleotides downstream from that of other sobemoviruses, leading to the lack of transmembrane segments at the N-termini of P2a/2a2b. In the current study, we resequenced this region for TRoV and LTSV. The hypothetical beginning of ORF1b was mapped as the beginning of ORF2a/2a2b for both TRoV and LTSV. Computional analysis revealed transmembrane segments at the N-termini of the TRoV and LTSV polyproteins.
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