Journal
MARINE BIOTECHNOLOGY
Volume 5, Issue 4, Pages 349-359Publisher
SPRINGER
DOI: 10.1007/s10126-002-0070-2
Keywords
marine mycelial fungi; Chaetomium indicum; b-1,3-glucanase; amylase; cellulase; N-acetyl-b-glucosaminidase
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Ninety fungal strains (42 species) isolated from marine habitats were studied for their ability to produce extracellular enzymes. Cultural filtrates of these strains were shown to contain a series of glycosidases (beta-glucosidases, N-acetyl-beta-glucosaminidases, beta-galactosidases alpha-mannosidases) and glucanases (1,3-beta-glucanases, amylases) which varied with habitat. The level of activity depended on the species of fungi. Several promising strains capable of producing both individual enzymes and a set of enzymes for splitting carbohydrate-containing compound have been isolated. Optimal conditions for growth of Chaetomium indicum and for biosynthesis of beta-1,3-glucanase were determined. beta-1,3-Glucanase was isolated using ion-exchange chromatography, ultrafiltration, and gel filtration. The presence of 2 enzyme forms was shown; both forms were exo-beta-1,3-glucanases.
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