Journal
BIOMOLECULAR ENGINEERING
Volume 20, Issue 4-6, Pages 139-147Publisher
ELSEVIER
DOI: 10.1016/S1389-0344(03)00040-6
Keywords
Dunaliella tertiolecta; Tetrasehnis suecica; alpha-tocopherol; chlorophyll-a; light limitation; nutrient limitation
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Batch experiments with Dunaliella tertiolecta and Tetraselmis suecica were performed to investigate alpha-tocopherol (alpha-T) production in time, in order to assess the effect of light availability per cell on the production of this antioxidant. In D. tertiolecta alpha-T content increased during growth, in other words, as the cell density increased and the light availability per cell decreased. In T suecica the pattern was different: alpha-T content was highest during the exponential phase, decreased significantly during the linear phase and increased again towards the end of the cultivation. Chlorophyll (chl-a) content of T suecica cells decreased after the exponential phase, instead of the expected increase typically observed in shade-adapted cells, suggesting that the culture was nutrient limited. An experiment with extra nutrients showed that chl-a and alpha-T content increased significantly during both the linear and the stationary phase when compared with values in nutrient-deficient conditions. No correlation between alpha-T and chl-a was observed. Our results indicate that diminished light availability does not limit alpha-T production in D. tertiolecta and T suecica, opening up the possibility of using high cell density, light-limited cultures for the production of this commercially interesting compound. (C) 2003 Elsevier Science B.V. All rights reserved.
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