Journal
ARCHIVES OF VIROLOGY
Volume 154, Issue 7, Pages 1151-1156Publisher
SPRINGER WIEN
DOI: 10.1007/s00705-009-0422-9
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Funding
- Guangzhou Science and Technology Program [2006Z1-E0011]
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Reverse genetics systems for generating recombinant influenza viruses are based on two different mechanisms for obtaining the 3' end of the viral RNA: one uses the self-cleaving hepatitis delta virus ribozyme (HDVR), and the other uses the murine RNA polymerase I (Pol I) terminator. In this study, we employed EGFP and Renilla luciferase reporter constructs to compare the efficiency of both methods. Our results indicate that the murine Pol I terminator was more efficient than the HDVR, which will be helpful in choosing an influenza virus rescue system, as well as in establishing other RNA virus rescue systems.
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