Journal
JOURNAL OF CELL BIOLOGY
Volume 162, Issue 1, Pages 47-57Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200303019
Keywords
dynein; flagella; Gas8; Gas11; TLTF
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Funding
- NCRR NIH HHS [P41 RR000592, RR 00592] Funding Source: Medline
- NIGMS NIH HHS [R37 GM055667, F32 GM 17901, F32 GM017901, GM 55667, R01 GM055667] Funding Source: Medline
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The dynein regulatory complex (DRC) is an important intermediate in the pathway that regulates flagellar motility. To identify subunits of the DRC, we characterized a Chlamydomonas motility mutant obtained by insertional mutagenesis. The pf2-4 mutant displays an altered waveform that results in slow swimming cells. EM analysis reveals defects in DRC structure that can be rescued by reintroduction of the wild-type PF2 gene. Immunolocalization studies show, that the PF2 protein is distributed along the length of the axoneme, where it is part of a discrete complex of polypeptides. PF2 is a coiled-coil protein that shares significant homology with a mammalian growth arrest-specific gene product (Gas11/Gas8) and a trypanosome protein known as trypanin. PF2 and its homologues appear to be universal components of motile axonemes that are required for DRC assembly and the regulation of flagellar motility. The expression of Gas8/Gas11 transcripts in a wide range of tissues may also indicate a potential role for PF2-related proteins in other microtubule-based structures.
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