Journal
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
Volume 13, Issue 13, Pages 2105-2108Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0960-894X(03)00377-9
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Esters and acyloxymethyl ethers of umbelliferone were evaluated as fluorogenic substrates for lipases and esterases with respect to stability and resistance to non-specific hydrolysis. lsobutyryloxymethyl ether le and pivaloxymethyl ether Id were found to be optimal substrates for enzyme assays, particularly with respect to HTS applications. (C) 2003 Elsevier Science Ltd. All rights reserved.
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