Journal
PHYSIOLOGICAL GENOMICS
Volume 14, Issue 2, Pages 95-106Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/physiolgenomics.00151.2002
Keywords
salivary glands; exocrine cells; secretion; mucins; postnatal development
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Funding
- NHLBI NIH HHS [HL-07126] Funding Source: Medline
- NIDCR NIH HHS [DE-10480, DE-13602] Funding Source: Medline
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NFS/N-sld mice harbor a spontaneous autosomal recessive mutation, sld (sublingual gland differentiation arrest) and histologically display attenuated mucous cell expression in sublingual glands (Hayashi et al. Am J Pathol 132: 187 - 191, 1988). Because altered serous demilune cell expression is unknown, we determined the phenotypic expression of this cell type in mutants. Moreover, we evaluated whether absence of glycoconjugate staining in 3-day-old mutant glands is related to disruption in apomucin gene expression and/or to posttranslational glycosylation events. Serous cell differentiation is unaffected, determined morphologically and by serous cell marker expression (PSP, parotid secretory protein; and Dcpp, demilune cell and parotid protein). Conversely, apical granules in atypical exocrine cells of mutant glands are PSP and mucin negative, but contain abundant SMGD (mucous granule marker). Age-related appearance of mucous cells is associated with expression of apomucin gene products, whereas SMGD expression is unaltered. Atypical cells thus appear specified to a mucous cell fate but do not synthesize mucin glycoproteins unless selectively induced postnatally, indicating the sld mutation disrupts apomucin transcriptional regulation and/or decreases apomucin mRNA stability.
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