Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 100, Issue 14, Pages 8378-8383Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1432871100
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- NIAID NIH HHS [U19 AI046132, AI39630, U19AI46132] Funding Source: Medline
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A significant number of self-reactive T cell clones escape thymic negative selection and are released into the periphery, where some are potentially pathogenic. The clonal expansion of self-reactive T cells is known to be limited during initial antigen encounter by apoptotic or anergic mechanisms, regulatory CD4(+) T cells, and cytokines. Here we report that superimposed on these mechanisms, during the evolution of autoimmunity in experimental autoimmune encephalomyelitis (EAE), CD8(+) T cells are induced, which fine-tune the peripheral self-reactive T cell receptor (TCR) repertoire. We assayed the myelin basic protein-reactive TCR repertoire in naive, EAE-recovered mice as well as EAE-recovered mice depleted of CD8+ T cells by TCRVbeta surface expression, complementarity-determining region 3 length distribution, and complementarity-determining region 3 sequencing analysis. In EAE-recovered mice, certain myelin basic protein-reactive CD4(+)Vbeta8.2(+) clones are significantly decreased and this decrease is not observed if CD8(+) T cells were depleted from these mice. The clones that persist in CD8(+) T cell-intact mice are highly diverse in contrast to the clones expanded in CD8(+) T cell-(.)depleted mice, which are dominated by the significant outgrowth of a few clones. Importantly, the T cell clones that expand in the absence of CD8(+) T cell control are enriched in potentially pathogenic self-reactive T cell clones capable of inducing EAE in vivo.
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