Journal
FEBS LETTERS
Volume 546, Issue 2-3, Pages 241-246Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(03)00579-9
Keywords
ABC transporter; membrane topology; site-directed mutagenesis; cysteine modification
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MacB is an ABC-type membrane protein that exports only macrolide compounds containing 14- and 15-membered lactones, cooperating with a membrane fusion protein, MacA, and a multifunctional outer membrane channel, TolC. We determined the membrane topology of MacB by means of site-specific competitive chemical modification of single cysteine mutants. As a result, it was revealed that MacB is composed of four transmembrane (TM) segments with a cytoplasmic N-terminal nucleotide binding domain of about 270 amino acid residues and a periplasmic large hydrophilic polypeptide between TM segments I and 2 of about 200 amino acid residues. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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