Journal
CELL
Volume 114, Issue 1, Pages 123-134Publisher
CELL PRESS
DOI: 10.1016/S0092-8674(03)00476-8
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Funding
- NIGMS NIH HHS [R37 GM29169, R01 GM55440] Funding Source: Medline
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During the ribosomal translocation, the binding of elongation factor G (EF-G) to the pretranslocational ribosome leads to a ratchet-like rotation of the 30S subunit relative to the 50S subunit in the direction of the mRNA movement. By means of cryo-electron microscopy we observe that this rotation is accompanied by a 20 Angstrom movement of the L1 stalk of the 50S subunit, implying that this region is involved in the translocation of deacylated tRNAs from the P to the E site. These ribosomal motions can occur only when the P-site tRNA is deacylated. Prior to peptidyl-transfer to the A-site tRNA or peptide removal, the presence of the charged P-site tRNA locks the ribosome and prohibits both of these motions.
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