Journal
BIOCHEMICAL JOURNAL
Volume 373, Issue -, Pages 313-318Publisher
PORTLAND PRESS LTD
DOI: 10.1042/BJ20030472
Keywords
Ca2+ stores; Ca2+ mobilization; endoplasmic reticulum; neurotransmitter release; nicotinic acid-adenine dinucleotide phosphate (NAADP); thapsigargin
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Funding
- NINDS NIH HHS [NS18710, NS39646] Funding Source: Medline
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Nicotinic acid-adenine dinucleotide phosphate (NAADP) is a recently described potent intracellular Ca2+ -mobilizing messenger active in a wide range of diverse cell types. In the present study, we, have investigated the interaction of NAADP with other Ca2+ -mobilizing messengers in the release of transmitter at the frog neuromuscular junction. We show, for the first time, that NAADP enhances neurosecretion in response to inositol 1,4,5-trisphosphate (IP3) cADP-ribose (cADPR) and sphingosine 1-phosphate (SlP), but not sphingosylphosphorylcholine. Thapsigargin was without effect on transmitter release in response to NAADP, but blocked the responses to subsequent application of IP3, cADPR and SIP and their potentiation by NAADP. Asynchronous neurotransmitter release may therefore involve functional coupling of endoplasmic reticulum Ca2+ stores with distinct Ca2+ stores targeted by NAADP.
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