Journal
FEBS LETTERS
Volume 547, Issue 1-3, Pages 228-232Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(03)00711-7
Keywords
actin; intermediate filament; lens; cytoskeleton; tropomodulin
Funding
- NEI NIH HHS [EY10814, F32 EY06982] Funding Source: Medline
Ask authors/readers for more resources
Tropomodulin (Tmod) is an actin filament pointed end capping protein found in the membrane skeleton of lens fiber cells. We demonstrate that Tmod4 is able to bind the lens-specific intermediate filament protein, filensin, in either co-sedimentation or solid phase binding assays in a saturable fashion, but with low affinity and stoichiometry. Furthermore, Tmod4 does not bind the 53 kDa rod domain of filensin, nor to CP49, the obligate assembly partner of filensin. Finally, the binding of filensin to Tmod4 does not inhibit the actin capping activity of Tmod4 in vitro, suggesting that the two functions are not mutually exclusive. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available