Skin backreflectance and microvascular system functioning at the action of osmotic agents

The in vivo back reflectance spectra of rat skin were measured by inter-dermal injection of glycerol and glucose solutions. The injection of glycerol solution caused a significant decrease in skin reflectance within the first few minutes, and the decreased reflectance was conserved for a long time period. The effect of glucose solution on skin optical clearing was also demonstrated. The decrease of reflectance was stronger than at glycerol injection but was conserved for a shorter period. For hyperosmotic solutions of glucose and glycerol the optical clearing of skin was coupled with stasis and dilation of microvessels in the area of application of the agent. To study the peculiarities of the blood microcirculation system the rat mesentery was used as an animal model. The effect of each agent on blood microcirculation had some distinctive features.