Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1007, Issue 1-2, Pages 77-84Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(03)00977-4
Keywords
food analysis; infant formulas; cereals; fruits; validation; vitamins
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A liquid chromatographic method for the separation and determination of several water-soluble vitamins of the B group is proposed. The procedure is based on the use of a new amide-based stationary phase, which avoids the need of using the ion-pair technique. leading to narrower peaks and a simpler mobile phase. Analyses were performed by gradient elution with acetonitrile-phosphate buffer as mobile phase and using a photodiode array detector. Specificity was demonstrated by the retention characteristics. UV spectra and by comparing the peak purity index with commercial standards. Linearity, precision, recovery and sensitivity were satisfactory. The vitamins are extracted from the baby food using a single digestion with hydrochloric acid followed by enzymatic digestion with taka-diastase. The method was successfully applied to the determination of nine vitamins: thiamine, riboflavin, nicotinamide, nicotinic acid, pyridoxine, pyridoxal, folic acid, cyanocobalamine and inosine in different baby foods as infant formulas, cereals and fruit products. Validation was performed using two certified reference materials. (C) 2003 Elsevier B.V. All rights reserved.
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