Journal
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
Volume 528, Issue 1-2, Pages 105-110Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0027-5107(03)00076-9
Keywords
G(2) chromosomal assay; lymphocytes; chromosomal aberrations; radiosensitivity
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The G(2) chromosomal radiosensitivity assay is a technically demanding assay. To ensure that it is reproducible in our laboratory, we have examined the effects of storage and culture conditions by applying the assay to a group of healthy controls and determined the extent of intra- and inter-individual variations. Nineteen different individuals provided one or more blood samples resulting in a total of 57 successful tests. Multiple cultures from a single blood sample showed no statistically significant difference in the number of chromatid type aberrations between cultures. A 24 h delay prior to culturing the lymphocytes did not significantly affect the induced G(2) score. Intra-individual variation was not statistically significant in seven out of nine individuals. Inter-individual variation was highly statistically significant (P < 0.001), indicating that there is a real difference between individuals in the response to radiation using this assay. (C) 2003 Elsevier Science B.V All rights reserved.
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