Journal
EUROPEAN JOURNAL OF PHARMACOLOGY
Volume 473, Issue 2-3, Pages 197-206Publisher
ELSEVIER
DOI: 10.1016/S0014-2999(03)01968-X
Keywords
cytochrome P450; downregulation; inflammation; serum mediator; cytokine
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Inflammation reduces activity and expression of hepatic cytochrome P450 (P450) and therefore diminishes drug biotransformation. This study aimed to identify the serum mediators triggered by a viral infection and an aseptic inflammation that downregulate P450 isoforms. Incubation of hepatocytes with serum from rabbits with a turpentine-induced inflammation or humans with a viral infection decreased the amount of cytochrome 1A1 (CYP1A1), 1A2 and 3A6 mRNA and apoproteins. By serum fractionation and immuno-neutralization, we showed that in the aseptic inflammation, interleukin-6 and, to a lesser degree, interleukin-1 are involved in the downregulation of all three isoforms. In serum from humans with a viral infection, interleukin-1, interleukin-6, interferon-gamma and tumour necrosis factor-a contribute to the downregulation of P450 isoforms. CYP1A1 and 1A2 are regulated by serum mediators at the transcriptional level, while the expression of CYP3A6 appears to be under the control of pre- and posttranscriptional mechanisms. (C) 2003 Elsevier B.V. All rights reserved.
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