Journal
FEMS MICROBIOLOGY LETTERS
Volume 224, Issue 2, Pages 255-259Publisher
OXFORD UNIV PRESS
DOI: 10.1016/S0378-1097(03)00453-1
Keywords
nitrogenase; Azospirillum brasilense; FeMoco; NifX
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Herbaspirillum seropedicae strains mutated in the nifX or orf1 genes showed 90% or 50% reduction in nitrogenase activity under low levels of iron or molybdenum respectively. Mutations in nifX or orf1 genes did not affect nif gene expression since a nifH::lacZ fusion was fully active in both mutants. nifX and the contiguous gene orf1 are essential for maximum nitrogen fixation under iron limitation and are probably involved in synthesis of nitrogenase iron or iron-molybdenum clusters. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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