Journal
FEMS MICROBIOLOGY LETTERS
Volume 224, Issue 2, Pages 291-297Publisher
OXFORD UNIV PRESS
DOI: 10.1016/S0378-1097(03)00450-6
Keywords
reverse transcriptase; retron; Salmonella enterica serovar Typhimurium LT2
Categories
Funding
- NIGMS NIH HHS [1R01GM63853] Funding Source: Medline
Ask authors/readers for more resources
Bacterial reverse transcriptase is responsible for the production of a small satellite DNA-RNA complex called multicopy single-stranded DNA (msDNA) that has been found in a wide variety of Gram-negative bacteria. Here we describe the isolation and characterization of a novel msDNA, msDNA-St85, from Salmonella enterica serovar Typhimurium LT2. We determined the nucleotide sequence of msDNA-St85 and the location of retron-St85 on the chromosome that is responsible for msDNA-St85 production by analyzing the complete genomic sequence of S. typhimurium LT2. It was found that the G+C content and the codon usage of retron-St85 were significantly different from those of the S. typhimurium genome, indicating that retron-St85 was probably acquired recently in this bacterium. This is the first report for identification of an msDNA in the genus Salmonella with the complete description and analysis of its retron. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available