Regulation of basal rhythmicity in protein kinase C activity by melatonin in immortalized rat suprachiasmatic nucleus cells

Melatonin phase shifts circadian rhythms of the neuronal firing rate and stimulates PKC activity at dusk (CT 10) and dawn (CT 23) in the rat suprachiasmatic nucleus (SCN) slice via activation of the MT2 melatonin receptor. We demonstrated that in the SCN2.2 cells basal PKC activity follows a rhythmic oscillation with an acrophase during the subjective dark phase (CT 14-CT 22) and nadirs during the subjective light phase at CT 2 and CT 10. Melatonin (0.01 - 10 nM, 10 min) significantly doubled basal PKC activity at CT 2 and CT 10, and decreased basal PKC activity at CT 6. We conclude that melatonin regulates the basal rhythm in PKC activity generated in SCN2.2 cells at the same periods of sensitivity observed in the native SCN. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.