Journal
ONCOGENE
Volume 22, Issue 31, Pages 4799-4806Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.onc.1206714
Keywords
CrkII; CrkIII; adaptor protein; ERK; MAP kinase
Funding
- NHLBI NIH HHS [HL22563, HL10165-01] Funding Source: Medline
- NIDDK NIH HHS [DK 41684] Funding Source: Medline
Ask authors/readers for more resources
As the role for adaptor proteins constantly proliferates, appreciation of their importance has never been higher. The Crk family of adaptor proteins is no exception. Currently comprising four members, v-Crk, CrkI, CrkII and Crk-like protein, we have introduced a fifth member, CrkIII. Cloned by the CORT technique, CrkIII is identical in sequence to CrkII until the second of its two SH3 domains, which is disrupted partway through and results in a nonfunctional domain and a unique C-terminal sequence. We have demonstrated the existence of native CrkIII at the message level using RT-PCR and RNAse protection assays, and at the protein level in mouse fibroblasts. We show that CrkII overexpression is capable of enhancing insulin-stimulated ERK activity, whereas CrkIII is not, thus partially characterizing a novel member of the Crk family and elucidating important effects mediated by the c-terminal SH3 domain.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available