Journal
MAGNETIC RESONANCE IN CHEMISTRY
Volume 41, Issue 8, Pages 589-598Publisher
JOHN WILEY & SONS LTD
DOI: 10.1002/mrc.1222
Keywords
NMR; H-1 NMR; C-13 NMR; gentamicin; antibiotics; separation
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The antibiotic agent gentamicin sulfate is a mixture of several structurally very similar aminoglycosides. The major components of gentamicin are gentamicin C1, C1a, C2 and C2a. These major components were separated by means of column chromatography. The H-1 and C-13 NMR spectra of the free bases and the corresponding sulfates were assigned using HMQC, HMBC and selective TOCSY experiments. Additionally, the H-1 and C-13 NMR spectra of the sulfates and hydrochlorides, respectively, of the minor components were assigned. The full assignment of all components of gentamicin is necessary for the quantitative analysis of batches of gentamicin. By means of coupled liquid chromatography-NMR, several different batches were studied, revealing different compositions of batches from various sources. Copyright (C) 2003 John Wiley Sons, Ltd.
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