Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 41, Issue 8, Pages 3893-3896Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.41.8.3893-3896.2003
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Seventeen Klebsiella pneumoniae clinical isolates carrying the bla(VIM-1) metallo-beta-lactamase gene were collected in the intensive care units of three hospitals in Athens, Greece, in 2002. They exhibited various carbapenem resistance levels (Etest MICs of imipenem ranged from 4 to 32 mug/ml). All isolates gave positive results by the imipenem-EDTA synergy Etest. The isolates were classified into four main types by pulsed-field gel electrophoresis; the majority of the isolates (5 and 10 isolates) belonged to two types. The bla(VIM-1) gene cassette was part of the variable region of a class I integron that also included aac6, dhftI, and aadA. This structure was carried by transferable plasmids.
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