Journal
BIOSENSORS & BIOELECTRONICS
Volume 18, Issue 8, Pages 1023-1029Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/S0956-5663(02)00225-7
Keywords
biochemical oxygen demand biosensor; Trichosporon cutaneum; Bacillus subtilis; sol-gel derived composite matrix
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A novel type of biochemical oxygen demand (BOD) biosensor was developed for water monitor, based on co-immobilizing of Trichosporon cutaneum and Bacillus subtilis in the sol-gel derived composite material which is composed of silica and the grafting copolymer of poly (vinyl alcohol) and 4-vinylpyridine (PVA-g-P(4-VP)). Factors that influence the performance of the resulting biosensor were examined. The biodegradable substrate spectrum could be expanded by the co-immobilized microorganisms. The biosensor prepared also exhibited good reproducibility and long-term stability. Good agreement was obtained between the results of the sensor BOD measurement and those obtained from conventional BOD5 method for water samples. (C) 2002 Elsevier Science B.V. All rights reserved.
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