Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 312, Issue 1-3, Pages 267-272Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0048-9697(03)00254-7
Keywords
HSP70; Chironomus tentans; copper; biomarkers
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In the present study, we investigated the induction of HSP70 proteins in Chironomus tentans in response to copper exposure. Larvae were exposed for 24 h to 0, 0.25, 0.5, 1.0 and 2.0 mg 1(-1) copper, concentrations that were previously shown to cause growth reduction or mortality in 10 d bioassays. The induction of HSP70 was evaluated by Western blotting, and we observed a significant increase in HSP70 proteins over controls at all tested concentrations. The results suggest that short-term laboratory exposures to chronically toxic but acutely sublethal copper concentrations induce HSP70 proteins in C. tentans larvae. (C) 2003 Elsevier Science B.V. All rights reserved.
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