Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 111, Issue 2, Pages 111-120Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0166-0934(03)00166-6
Keywords
human immunodeficiency virus type 1; DNA; yeast; entry; env; chimeric
Funding
- NIAID NIH HHS [AI36219, AI49170] Funding Source: Medline
- NICHD NIH HHS [N01-HD-0-3310-502-02] Funding Source: Medline
Ask authors/readers for more resources
A recent shift from studies on a few subtype B laboratory human immunodeficiency virus type 1 (HIV-1) clones to analyses of extremely diverse primary HIV-1 isolates from different subtype requires the development of a rapid and generic cloning technique. This report describes the use of gap repair/recombination in yeast to shuttle env, gag, and pol genes from diverse HIV-1 subtypes into a DNA vector that can be amplified in bacteria and can express the gene of interest in mammalian cells. These diverse HIV-1 genes have also been introduced into an infectious clone to produce chimeric viruses that are useful for studies on drug susceptibility, receptor binding and fitness. (C) 2003 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available