Journal
BIOTECHNOLOGY LETTERS
Volume 25, Issue 16, Pages 1345-1349Publisher
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1024988605769
Keywords
calcium flux; Catharanthus roseus; hairy roots; indole alkaloids; serpentine
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Treatment of Catharanthus roseus hairy roots with antagonists, like verapamil and CdCl2, that block the Ca2+ flux across the plasma membrane enhanced the total alkaloid content by 25% and their secretion 10 times. The specific Ca2+ chelator, EGTA, stimulated 90% of the total alkaloid secretion. Treatment with inhibitors of intracellular Ca2+ movement, like TMB-8 and trapsigargin, enhanced the total alkaloid content by 74% and their secretion into the culture media by 4- to 6-fold. The results suggest that an inhibition of external and internal Ca2+ fluxes induces an increase in the indole alkaloid accumulation and secretion in C. roseus hairy roots.
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